Journal: Scientific Reports

Article Title: Maternal Bacillus probiotic regulates offspring growth and immunity via spleen IGF-1/mTOR and FOXO1/IL-10 pathways

doi: 10.1038/s41598-026-38412-y

Figure Lengend Snippet: Serum levels of growth hormone (GH) and insulin-like growth factor-1 (IGF-1) in male S. albino mice offspring at postnatal day 28 following maternal Bacilli supplementation at different gestational stages. Data are presented as mean ± SE (n = 6 /group). Statistical comparisons were performed using one-way ANOVA followed by Tukey’s post hoc test. Groups sharing different superscript letters differ significantly at p < 0.05. Abbs: GD0, gestation day 0; GD8, gestation day 8; GD16, gestation day 16.

Article Snippet: According to Baxter and Martin mechanism of analysis, serum IGF-1 (6 samples/group) levels were analyzed following the protocol of the manufacturer-specific mouse IGF-1 ELISA kit (Catalog No. CSB-E04581m, Detection range: 0.156–10 ng/mL and sensitivity: less than 0.151 ng/mL, CUSABIO Company, USA).

Techniques:

Journal: Scientific Reports

Article Title: Maternal Bacillus probiotic regulates offspring growth and immunity via spleen IGF-1/mTOR and FOXO1/IL-10 pathways

doi: 10.1038/s41598-026-38412-y

Figure Lengend Snippet: ( A–D ) Immunohistochemical staining of the IGF-1 and FOXO1 markers in spleen sections from male offspring of S. albino mice following probiotic supplementation. ( A ) The GD0 and GD8 groups exhibited increased IGF-1 expression (brown color) in the periarteriolar lymphoid sheath (PALS), germinal centers, marginal zones, and stromal cells of the red pulp compared to the control. ( B ) The FOXO1 marker exhibited strong nuclear staining (brown colour) in the control group, whereas all Bacillus-treated groups (GD0, GD8 and GD16) showed a faint nuclear FOXO1 expression. IHC splenic tissues were examined under light microscopy at × 200 magnification; scale bar = 100 µm. ( C and D ) graphs: The integrated density percentage analysis of immunostaining for IG-1 and FOXO 1, respectively; according to the statistical analysis, one-way ANOVA followed by Tukey’s post hoc test. Data are presented as mean ± SE; groups sharing different superscript letters differ significantly at p < 0.05. Abbs : IGF-1 , Insulin-like Growth Factor-1; GD0 , gestation day 0; GD8 , gestation day 8; GD16 , gestation day 16.

Article Snippet: According to Baxter and Martin mechanism of analysis, serum IGF-1 (6 samples/group) levels were analyzed following the protocol of the manufacturer-specific mouse IGF-1 ELISA kit (Catalog No. CSB-E04581m, Detection range: 0.156–10 ng/mL and sensitivity: less than 0.151 ng/mL, CUSABIO Company, USA).

Techniques: Immunohistochemical staining, Staining, Expressing, Control, Marker, Light Microscopy, Immunostaining

Journal: Data in Brief

Article Title: Data of small molecule compounds and insulin-like growth factor-1 (IGF-1) detected in Deer Antler Velvet of Malayan Deer ( Cervus timorensis )

doi: 10.1016/j.dib.2025.112339

Figure Lengend Snippet: Standard curve of IGF-1 ELISA assay log 10 (IGF-1) ng/ml).

Article Snippet: Data collection , Data of small molecules in water (WE), 70 % ethanol (70 % EE) and absolute ethanolic extracts (AEE) of DAV obtained from the antler of local male Malayan deer ( Cervus timorensis ) species were acquired using Agilent 1290 Infinity Liquid Chromatography system coupled to Agilent 6520 Accurate-Mass Quadrupole Time-of-Flight mass spectrometer with dual electrospray ionization (ESI) source. Detection of IGF-1 was performed using commercially available Elisa Kit (CSB-E12644De; CUSABIO)..

Techniques: Enzyme-linked Immunosorbent Assay